primary antibody against glial fibrillary acidic protein  (Cell Signaling Technology Inc)

Journal: Frontiers in Pharmacology

Article Title: Integrating network pharmacology and transcriptomics to reveal the therapeutic effect of Long Mu Ning Xin Decoction on attention-deficit/hyperactivity disorder by regulating cAMP and PI3K/AKT pathways

doi: 10.3389/fphar.2026.1744709

Figure Lengend Snippet: Effects of LMNXD on the relative expression of GFAP and DRD1 positive Area% in the hippocampus and PFC of rats and BDNF protein in the striatum. (A) Immunofluorescence staining of GFAP (Scale: 100um). (B) Immunohistochemical staining of DRD1 (Scale: 200um). (C) Western blotting analysis image of BDNF in the striatum. (D) Relative GFAP positive area%. (E) Relative DRD1 positive area%. (F) Relative expression of BDNF proteins. Data are expressed as mean ± standard deviation, n = 3 in immunohistochemistry and immunofluorescence, n = 6 in Western blot. Compared with the WKY group, # p < 0.05, ## p < 0.01. Compared with the SHR group, * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Primary antibodies against glial fibrillary acidic protein (GFAP, 60190-1-Ig), dopamine deceptor D1 (DRD1, 17934-1-Ap), and BDNF (66292-1-Ig) were obtained from Proteintech (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Staining, Immunohistochemical staining, Western Blot, Standard Deviation, Immunohistochemistry

Journal: Journal of Traditional and Complementary Medicine

Article Title: Elucidating the mechanism of traditional Chinese medicine formula ( Yifei-sanjie Pill ) in alleviating the chemobrain based on network pharmacology and experimental verification

doi: 10.1016/j.jtcme.2024.11.008

Figure Lengend Snippet: YFSJ reverses DDP-induced brain tissue damage. (A) Results of Nissl staining of the mouse hippocampus. The black arrow depicts the degenerative changes in the neurons in the DG sectors. Scale bar = 100 μm. (B) Effects of FJB staining on the hippocampus. Green fluorescence indicates degenerated neurons in the DG sectors. Scale bar = 100 μm. (C) Results of LFB staining of the hippocampus. The blank areas were stained to indicate myelin loss in neurons in the DG sectors. Scale bar = 200 μm. (D) GFAP and Iba1 immunostaining in the hippocampus. Merged overview of the relevant DG sectors with GFAP in red, Iba1 in green, and nuclear counterstaining with DAPI in blue. Scale bar = 50 μm.

Article Snippet: Primary antibodies against GFAP (Cat. #80788T), Iba1 (Cat. #17198T), Occludin (Cat. #91131T), Claudin-5 (Cat. #49564S), GPX4 (Cat. #59735S), and GAPDH (Cat. #5174T) and secondary antibodies anti-rabbit (Cat. #7074P2, 8889S and 4412S) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Staining, Fluorescence, Immunostaining

Journal: Neural Regeneration Research

Article Title: Positive impact of indicaxanthin from Opuntia ficus-indica fruit on high-fat diet–induced neuronal damage and gut microbiota dysbiosis

doi: 10.4103/NRR.NRR-D-23-02039

Figure Lengend Snippet: Indicaxanthin ameliorates brain inflammation induced by HFD. (A) Transcription levels of iNOS, TNF-α, and IL-6 determined by real-time PCR in the brain. (B) Brain expression of COX-2 and iNOS. (C) Densitometric analysis of COX-2 and iNOS protein levels normalized to β-actin levels. (D) Brain expression of cytosolic p65 and nuclear p65. (E) Densitometric analysis of cytosolic p65 protein levels normalized to β-actin levels and nuclear p65 levels normalized to laminin B levels. (F) Representative images of GFAP-positive cells (red) on the surface. Hoechst staining was used to label the nuclei (blue) (microscope magnification 10×); scale bars: 100 μm. (G) Percentage of GFAP-positive cells. (H, I) Plasma circulating levels of TNF-α (H) and IL-1β (I). Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; ### P < 0.001, vs. HFD-fed mice. COX-2: Cyclooxygenase-2; GFAP: glial fibrillary acidic protein; HFD: high-fat diet; IL-6: interleukin-6; Ind: indicaxanthin; iNOS: inducible nitric oxide synthase; PCR: polymerase chain reaction; STD: standard diet; TNF-α: tumor necrosis factor-α.

Article Snippet: Next, the sections were incubated with a mouse primary antibody against glial fibrillary acidic protein (GFAP) (1:300, Cell Signaling Technology, Danvers, MA, USA, Cat# 3670, RRID: AB_10693476) at 4°C overnight and then with an anti-mouse IgG-Alexa Fluor 594 secondary antibody (goat, 1:300, Invitrogen, Cat# A-11005, RRID: AB_2534073) for 2 hours at room temperature.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Staining, Microscopy, Clinical Proteomics, Polymerase Chain Reaction